ABSTRACT
Objective To establish of a close loop blood information system forreal time monitoring in the entire process of clinical blood transfusion.Methods In accordance with files and laws in clinical blood transfusion and combining the work in blood transfusion department with the actual needs of clinical blood transfusion,the blood transfusion department in Shaanxi Provinical People's Hospital developed the close-loop blood information system with Skynet Software Co.Ltd.Results The close-loop blood information system was achieved informational management patternthat was detailed information from blood products arrival to departure and whole process of evaluation transfusion effect.The link of Hospital Information Manage System (HIS),Laboratory Information System (LIS) and blood information system was completed with the system.The system realized real-time and accurate data collection and monitoring.The system achieved a closed-loop management of the entire process of clinical blood transfusion.Conclusion By using theclose loop blood information system,the quality of medical services can be improved,the work productivity can be increased,and the clinical blood transfusion can be rationalized.
ABSTRACT
The kaurenoic acid-type diterpenoids in Acanthopanacis Cortex have been reported to be the major active components. However, the diterpenoids are present as position isomers that exacerbate the challenges in obtaining standards compounds. Little work has been done on the quantitative analysis of the diterpenoids in the herb. In the present study, two diterpenoid isomers ent-16βH,17-isovalerate-kauran-19-oic acid (1) and ent-16βH,17-methyl butanoate-kauran-19-oic acid (2) with high purity were separated by analytical HPLC, followed by recrystallization in acetone. Furthermore, an HPLC-ELSD method was developed and validated for simultaneous determination of 1 and 2 in 9 batches of Acanthopanacis Cortex samples. The HPLC separation and quantification was achieved in 40 min using an Agela Promosil C column eluted with a gradient of water and acetonitrile. The calibration curves showed good linearity (r ≥ 0.999 9) within the test ranges. The LOD ranged from 0.407 2 to 0.518 0 μg and LOQ ranged from 1.018 0 to 1.295 0 μg. The precisions (%RSD) were within 1.47% for the two isomers. The recovery of the assay was in the range of 98.78%-99.11% with RSD values less than 2.76%. It is the first time to establish a quantitative HPLC method for the analysis of the bioactive kaurenoic acid isomers in the herb.
Subject(s)
Chromatography, High Pressure Liquid , Diterpenes , Chemistry , Drugs, Chinese Herbal , Chemistry , Eleutherococcus , Chemistry , Isomerism , Plant Roots , ChemistryABSTRACT
Objective: Using fast centrifugal partition chromatography (FCPC) to remove (-)-bicuculline from Corydalis Decumbentis Rhizoma total alkaloids. Methods: The technological parameters of orthogonal experiment of FCPC was optimized by flow rate, rotation speed, and loading sample size. The optimized process was compared with the solvent extraction method, and the linear amplification experiments were carried out using the optimal process. Results: The optimized parameters were as follows: rotation speed was 1200 r/min, flow rate was 4 mL/min, and loading sample size was 80 mg/mL. The new prepared alkaloids fraction of Corydalis Decumbentis Rhizoma using amplification experiments has > 94.0% removed rate of BI and >85.0% recovery of the other alkaloids, which were higher than those of the solvent extraction method. This simple method with good repeatability could be completed in a short time. Conclusion: The simple and fast method could improve the safety of Corydalis Decumbens Rhizoma total alkaloids, and provide the industrialization of non-toxic total alkaloids.